{"title":"Specific Use Pre-Pour Agar and Slants","description":"\u003cp\u003ePre-Poured Water agar for use with wild cloning, Antibiotic agar for soil samples and PDYA slants for long term culture storage!\u003c\/p\u003e","products":[{"product_id":"water-agar-petri-dishes-for-wild-fungal-cloning-and-mushroom-mycelium-cultivation","title":"Water Agar Petri Dishes for Wild Fungal Cloning and Mushroom Mycelium Cultivation (10pk)","description":"\u003cp\u003e\u003cspan\u003ePack of 10 shrink wrapped plates.\u003cbr\u003eParafilm can be added at a small cost.\u003cbr\u003e\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003cspan\u003eRequirements : SAB\/\u003c\/span\u003e\u003ca href=\"https:\/\/mycopunks.com\/products\/flow-hood\"\u003e\u003cspan\u003eFlow Unit\u003c\/span\u003e\u003c\/a\u003e\u003cspan\u003e \u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003eWater agar plates have no nutrients, they provide a platform and moisture for your wild mycelium to grow upon whist denying bacteria and yeasts a food source. When successful you will see sparse growth from your piece of cloned fungi. Once growth is enough, transfer to a wide spectrum media plate like MEYA or PDYA to feed your new healthy sample!\u003c\/p\u003e","brand":"MycoPunks","offers":[{"title":"No","offer_id":40739443703999,"sku":"WATERAGAR10","price":14.0,"currency_code":"GBP","in_stock":true},{"title":"Yes","offer_id":55466340745597,"sku":"WATERAGAR10_PARA","price":15.5,"currency_code":"GBP","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0542\/6721\/6063\/files\/Agar.png?v=1741474713"},{"product_id":"antibiotic-agar-for-wild-cultures-clones","title":"Antibiotic Sabouraud Dextrose Agar with Chloramphenicol (10pk)","description":"\u003cp\u003e\u003cspan\u003ePack of 10 shrink wrapped plates.\u003cbr\u003eParafilm can be added at a small cost.\u003cbr\u003eRequirements : SAB\/\u003ca href=\"https:\/\/mycopunks.com\/products\/flow-hood\"\u003eFlow Unit\u003c\/a\u003e \u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003eAfter a bunch of requests we've been trying to find a suitable antibiotic agar.\u003c\/p\u003e\n\u003cp\u003eWe've been working with some boffins in the \"lab stuff\" sector and we are happy to offer a really good option. \u003c\/p\u003e\n\u003cp\u003eSabouraud Dextrose Agar with Chloramphenicol is a dextrose peptone based agar with antibiotic c\u003cspan\u003ehloramphenicol included. \u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003ePlease note, this is not a cure for contamination. It will kill off bacteria giving mycelium a fighting chance, it doesn't kill mold or yeast.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eThis product should be used only after giving consideration to the effects antibiotics have in the wider environment. E.g. How overuse of antibiotics can encourage antibiotic resistant strains of bacteria. \u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eAlways read the labels.\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e\u003c\/p\u003e","brand":"MycoPunks","offers":[{"title":"No","offer_id":55466124247421,"sku":"ANTIBIOTICAGAR-1","price":20.0,"currency_code":"GBP","in_stock":true},{"title":"Yes","offer_id":55466124280189,"sku":"ANTIBIOTICAGAR-2","price":21.5,"currency_code":"GBP","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0542\/6721\/6063\/files\/7DD9BE27-F459-4858-99CE-EC358E02925A.jpg?v=1741471374"},{"product_id":"pdya-slants-for-long-term-culture-storage","title":"PDYA Slants for long term culture storage (5pk)","description":"\u003cp dir=\"ltr\"\u003e\u003cspan\u003e5 PDYA Agar Slants: what’s this all about?\u003c\/span\u003e\u003c\/p\u003e\n\u003cp dir=\"ltr\"\u003e\u003cspan\u003eIf you’re experimenting with lots of different mushroom strains at the same time, you might find that you end up with a bit much on your plate at once: all your agar plates are ready to go at the same time, and there’s only so many hours in the day (and only so much space in your monotubs)!\u003c\/span\u003e\u003c\/p\u003e\n\u003cp dir=\"ltr\"\u003e\u003cspan\u003eYou \u003c\/span\u003e\u003cspan\u003ecould\u003c\/span\u003e\u003cspan\u003e try and slow the growth on your plates a little by putting them in the fridge, but you’ll find that the mycelium will, over time, take up all the available nutrients in the agar, and eventually all of the available moisture. You might even have mushrooms beginning to grow straight on the plate: yeah, it’s a fun novelty but it’s not actually that useful, and is a bit of a waste of your resources.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp dir=\"ltr\"\u003e\u003cspan\u003eAnd that exact scenario is what agar slants were designed to solve! It’s the tried-and-tested \u003c\/span\u003e\u003ca href=\"https:\/\/mycopunks.com\/products\/potato-dextrose-agar-pda-petri-dishes-for-mushroom-mycelium-cultivation?variant=55459730293117\"\u003e\u003cspan\u003epotato dextrose and yeast extract\u003c\/span\u003e\u003c\/a\u003e\u003cspan\u003e combo that everyone knows and loves, with a slightly craftier form. We angle the vials as the agar is poured that’s why it’s called a slant) to create a usable surface area but with a much greater depth that a standard shallow agar plate. There’s the same amount of nutritious agar, but it takes longer for the mycelium to access, which means it’ll stay alive for much longer. The slanted angle also means that any condensation runs down the side of the slant and doesn’t just pool on top of your mycelium, which makes this particular form factor absolutely ideal for long-term fridge storage. Use these, and you can have a whole load of mushroom genetics ready to grow, at the time that suits you best. Science: it’s for everyone.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp dir=\"ltr\"\u003e\u003cspan\u003eYou will need to be using a SAB at the \u003c\/span\u003e\u003cspan\u003eabsolute minimum\u003c\/span\u003e\u003cspan\u003e (and that’s still gambling), but we’d recommend a \u003c\/span\u003e\u003ca href=\"https:\/\/mycopunks.com\/products\/mycopunks-mini-flow-unit-h14-hepa-for-mushroom-cultivation\"\u003e\u003cspan\u003eFlow unit\u003c\/span\u003e\u003c\/a\u003e\u003cspan\u003e for guaranteed results.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp dir=\"ltr\"\u003e\u003cspan\u003e✅ Maximum surface area\/depth ratio for long-term storage\u003c\/span\u003e\u003c\/p\u003e\n\u003cp dir=\"ltr\"\u003e\u003cspan\u003e✅ Five of them! \u003c\/span\u003e\u003c\/p\u003e\n\u003cp dir=\"ltr\"\u003e\u003cspan\u003e✅ Excellent for keeping in a fridge\u003c\/span\u003e\u003c\/p\u003e\n\u003cp dir=\"ltr\"\u003e\u003cspan\u003e✅ Could likely use it as a sex toy if you felt like it\u003c\/span\u003e\u003c\/p\u003e\n\u003cp dir=\"ltr\"\u003e\u003cspan\u003e✅ Mind you that’s probably true about most things\u003c\/span\u003e\u003c\/p\u003e\n\u003cp dir=\"ltr\"\u003e\u003cb\u003e\u003c\/b\u003e\u003cspan\u003e\u003cmeta charset=\"utf-8\"\u003e\n\u003cb id=\"docs-internal-guid-98fcae51-7fff-9a99-736a-bfa959616df3\"\u003e✅ \u003c\/b\u003eBuy more? Pay less!\u003c\/span\u003e\u003c\/p\u003e\n\u003cp dir=\"ltr\"\u003e\u003cstrong\u003eStorage:\u003c\/strong\u003e\u003cb\u003e\u003c\/b\u003e\u003c\/p\u003e\n\u003cul\u003e\n\u003cli dir=\"ltr\" role=\"presentation\"\u003e\u003cspan\u003eSomewhere cool, dark, and dry. Honestly, just keep them in the fridge, that’s what they’re designed for.\u003c\/span\u003e\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp dir=\"ltr\"\u003e\u003cspan\u003eAll of our agar products are covered by our \u003c\/span\u003e\u003ca href=\"https:\/\/help.mycopunks.com\/en-GB\/articles\/returns-and-refunds-231449\"\u003e\u003cspan\u003e60 Day Returns Policy\u003c\/span\u003e\u003c\/a\u003e\u003cspan\u003e, same as our grain and substrate!\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e\u003c\/p\u003e","brand":"MycoPunks","offers":[{"title":"5 x Standard","offer_id":43445164114155,"sku":"5-PDYA-Slants","price":9.99,"currency_code":"GBP","in_stock":true},{"title":"5 x with hardwood","offer_id":43445164146923,"sku":"5-PDYA-Slants-Wood","price":11.99,"currency_code":"GBP","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0542\/6721\/6063\/files\/MycoPunks_PDYA_Slants_for_long_term_culture_storage_5pk.jpg?v=1751454972"},{"product_id":"easy-af-agar-pots","title":"Basics: Easy AF Agar Pots 4pk","description":"\u003cp\u003eThis might be one of the coolest products we've added in a while.\u003c\/p\u003e\n\u003cp\u003eWe asked our Discord community what we should call these so out of respect for our unhinged customer base I'll include the names they suggested at the bottom.\u003c\/p\u003e\n\u003cp\u003eThe product itself isn't anything particularly new. It's basically a modified pp5 pot with high nutrient agar. It has a Microppose injection port and filter to allow gas exchange.\u003c\/p\u003e\n\u003ch3\u003eWhy is it cool?\u003c\/h3\u003e\n\u003cp\u003e\u003cstrong\u003eGerminating spores\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eFirstly it gets you using agar if you've never tried it, you can inject a few drops of spore solution through the port and watch it grow out on the agar, the high nutrient mix shows contamination.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eLiquid Culture\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eThis is where it gets really cool. The typical process for liquid creation is to create sterile liquid culture media and transfer your mycelium from agar to the LC once the plate has colonised nicely. This requires some aseptic technique. At a bare minimum a still air box.\u003c\/p\u003e\n\u003cp\u003eI thought recently, why not do it the other way around?\u003c\/p\u003e\n\u003cp\u003eSo we added 20ml of sterile LC media to the pot after the mycelium had established. Sure enough, 2 days later we had mycelium growing in the liquid on the surface of the agar. We shook it up, then drew up the liquid in a syringe and transferred it back into sterile jar of media. \u003c\/p\u003e\n\u003cp\u003eWithout any aseptic technique we'd made a liquid culture from spores using the correct(ish) method using agar.\u003c\/p\u003e\n\u003cp\u003eI'm sure someone will tell me ShitBum_2000 on the Shroomery did this first in '99, but until then I'm claiming this as \u003cstrong\u003eMYCOPUNKS LC KETCHUP POT TEK \u003csup\u003etm\u003c\/sup\u003e\u003c\/strong\u003e.\u003c\/p\u003e\n\u003cp\u003eIt doesn't stop there...\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eNeedle Biopsy Cloning\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eWe've successfully cloned mushrooms using a large gauge needle to biopsy mycelium from a mushroom and put it directly through the injection port onto agar.\u003c\/p\u003e\n\u003cp\u003eThis could be especially cool for collecting mycelium in the field for cleaning up later.\u003c\/p\u003e\n\u003cp\u003eSo now we've cloned, germinated and created liquid culture with no still air box.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eCustomer name suggestions (mental) - sorry in advance:\u003c\/strong\u003e\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003eMushwombs (actually quite clever)\u003c\/li\u003e\n\u003cli\u003eCumpots\u003c\/li\u003e\n\u003cli\u003e\u003cspan\u003ePotsMyco \u003c\/span\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cspan\u003eCum Chalice\u003c\/span\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cspan\u003edonor dumps\u003c\/span\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cspan\u003ePunkplates\u003c\/span\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cspan\u003es(PunkPots)\u003c\/span\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cspan\u003ePots of Glory - With Holes\u003c\/span\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cspan\u003eFractal Fuel\u003c\/span\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cspan\u003eSplooge Pots\u003c\/span\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cspan\u003ePunk Holes - \"ready for a quick prick\"\u003c\/span\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cspan\u003eEZ-Clean plates\u003c\/span\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cspan\u003eSporegasms\u003c\/span\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cspan\u003eGrowgurt\u003c\/span\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cspan\u003ePot luck\u003c\/span\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cspan\u003eMycobrew\u003c\/span\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cspan\u003ePetit Agars\u003c\/span\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cspan\u003eSperm Sample Pot\u003c\/span\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cspan\u003eSpunknoculation Pots\u003c\/span\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cspan\u003eCum Buckets\u003c\/span\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cspan\u003eLil Chubs\u003c\/span\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cspan\u003eCM pots\u003c\/span\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cspan\u003eUncle Chris’s gelatinous prick vessel.\u003c\/span\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cspan\u003eSporeplay\u003c\/span\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cspan\u003eShank-n-wank tanks\u003c\/span\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cspan\u003eKumpot\u003c\/span\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cspan\u003eMycoMuffs\u003c\/span\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cspan\u003estab and squirt pot\u003c\/span\u003e\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"MycoPunks","offers":[{"title":"Default Title","offer_id":56190586814845,"sku":"AgarPots4pk","price":8.0,"currency_code":"GBP","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0542\/6721\/6063\/files\/EasyAF_Agar_Pots.webp?v=1751016140"}],"url":"https:\/\/mycopunks.pl\/collections\/specific-use-pre-pour-agar-and-slants.oembed","provider":"MycoPunks","version":"1.0","type":"link"}